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human osteopontin duo set elisa  (R&D Systems)


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    R&D Systems human osteopontin duo set elisa
    Human Osteopontin Duo Set Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opn+duo+set+elisa/Human+Osteopontin+(OPN)+DuoSet+ELISA/pm33452981-60-27-32
    Average 99 stars, based on 73 article reviews
    human osteopontin duo set elisa - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme
    Article Snippet: .. OPN levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) 48 h after transfection using the OPN duo set ELISA (DY1433, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Murine GL261 (RRID: CVCL_Y003) and human HeLa (RRID: CVCL_0030) cell lines were purchased from the National Cancer Institute (Bethesda, MD, USA) and ATCC (Manassas, VA, USA), respectively.

    Article Title: MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme.
    Article Snippet: .. OPN levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) 48 h after transfection using the OPN duo set ELISA (DY1433, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Murine GL261 (RRID: CVCL_Y003) and human HeLa (RRID: CVCL_0030) cell lines were purchased from the National Cancer Institute (Bethesda, MD, USA) and ATCC (Manassas, VA, USA), respectively.

    Transfection:

    Article Title: MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme
    Article Snippet: .. OPN levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) 48 h after transfection using the OPN duo set ELISA (DY1433, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Murine GL261 (RRID: CVCL_Y003) and human HeLa (RRID: CVCL_0030) cell lines were purchased from the National Cancer Institute (Bethesda, MD, USA) and ATCC (Manassas, VA, USA), respectively.

    Article Title: MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme.
    Article Snippet: .. OPN levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) 48 h after transfection using the OPN duo set ELISA (DY1433, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Murine GL261 (RRID: CVCL_Y003) and human HeLa (RRID: CVCL_0030) cell lines were purchased from the National Cancer Institute (Bethesda, MD, USA) and ATCC (Manassas, VA, USA), respectively.



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    human osteopontin duo set elisa  (R&D Systems)
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    R&D Systems human osteopontin duo set elisa
    Human Osteopontin Duo Set Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiR-181 family regulates <t>OPN.</t> ( A ) Schema showing the derivation of M0 and M2 polarized macrophages that are the dominant phenotype of macrophages in gliomas. ( B ) Transient transfection of M0 or M2 macrophages on day 6 for 48 h with either non-targeting control (NC) or miR-181a/b/c/d-5p mimics. MiR overexpression levels were confirmed by qRT-PCR. ( C ) Analysis of the resulting cells revealed that overexpression of miR-181a/b/c/d mimics resulted in a decrease in OPN gene expression at the RNA level (qRT-PCR) and ( D ) the protein level <t>(ELISA).</t> Data were normalized against the levels of U6 small RNA for all qRT-PCR experiments. The data represent the mean ± SD ( n = 3) in three independent experiments. Data is cumulative of 3 independent experiments run in triplicates. Statistical significance was determined using an unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Opn Duo Set Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opn+duo+set+elisa/Human+Osteopontin+(OPN)+DuoSet+ELISA/pmc07765845-134-18-23
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    MiR-181 family regulates OPN. ( A ) Schema showing the derivation of M0 and M2 polarized macrophages that are the dominant phenotype of macrophages in gliomas. ( B ) Transient transfection of M0 or M2 macrophages on day 6 for 48 h with either non-targeting control (NC) or miR-181a/b/c/d-5p mimics. MiR overexpression levels were confirmed by qRT-PCR. ( C ) Analysis of the resulting cells revealed that overexpression of miR-181a/b/c/d mimics resulted in a decrease in OPN gene expression at the RNA level (qRT-PCR) and ( D ) the protein level (ELISA). Data were normalized against the levels of U6 small RNA for all qRT-PCR experiments. The data represent the mean ± SD ( n = 3) in three independent experiments. Data is cumulative of 3 independent experiments run in triplicates. Statistical significance was determined using an unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Cancers

    Article Title: MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme

    doi: 10.3390/cancers12123813

    Figure Lengend Snippet: MiR-181 family regulates OPN. ( A ) Schema showing the derivation of M0 and M2 polarized macrophages that are the dominant phenotype of macrophages in gliomas. ( B ) Transient transfection of M0 or M2 macrophages on day 6 for 48 h with either non-targeting control (NC) or miR-181a/b/c/d-5p mimics. MiR overexpression levels were confirmed by qRT-PCR. ( C ) Analysis of the resulting cells revealed that overexpression of miR-181a/b/c/d mimics resulted in a decrease in OPN gene expression at the RNA level (qRT-PCR) and ( D ) the protein level (ELISA). Data were normalized against the levels of U6 small RNA for all qRT-PCR experiments. The data represent the mean ± SD ( n = 3) in three independent experiments. Data is cumulative of 3 independent experiments run in triplicates. Statistical significance was determined using an unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: OPN levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) 48 h after transfection using the OPN duo set ELISA (DY1433, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Transfection, Control, Over Expression, Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay